Composite

Part:BBa_K2683027:Design

Designed by: Kristi Turton   Group: iGEM18_Lethbridge   (2018-10-10)


Elongation Factor- Thermo Stable (EF-Ts) in pSB1C3


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 88
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The subparts and coding sequence of this part is optimized for use in E.coli .

Source

https://parts.igem.org/Part:BBa_K2443028

References

[1] Zhang, Y., Sun, V., and Spremulli, L. (1997) Role of domains in Escherichia coli and mammalian mitochondrial elongation factor Ts in the interaction with elongation factor Tu. Journal of Biological Chemistry. 272, 21956-21963.